The mouse monoclonal antibody E11 recognizes an extracellular epitope of CD35 (CR1), a type I transmembrane glycoprotein expressed on granulocytes, monocytes, B cells, folicular dendritic cells, erythrocytes, NK and T cell subsets, as well as e.g. on glomerulal podocytes.
Kreuzreaktivität (Details)
Human, Non-Human Primates
Aufreinigung
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Immunogen
Acute monocytic leukemia cells and normal blood monocytes
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C
Informationen zur Lagerung
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Hogg, Ross, Jones, Slusarenko, Walport, Lachmann: "Identification of an anti-monocyte monoclonal antibody that is specific for membrane complement receptor type one (CR1)." in: European journal of immunology, Vol. 14, Issue 3, pp. 236-43, (1984) (PubMed).
Complement C3b/C4b receptor 1 (Knops blood group),CD35 (complement receptor 1, CR1) is a monomeric multiple modular cell surface glycoprotein which serves as receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. It is expressed mainly on the surface of granulocytes, monocytes, erythrocytes, B cells and folicular dendritic cells. Besides its role in complement cascade, CD35 is involved in blocking BCR-induced proliferation and the differentiation of B cells to plasmablasts and their Ig production.,CR1, KN, C3BR, C4BR