VASP Antikörper

Produktnummer ABIN2745577

Der Kaninchen Polyklonal anti-VASP Antikörper (ABIN2745577) detektiert spezifisch VASP in WB, IP und ICC. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für VASP Antikörper (ABIN2745577)

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser VASP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Produktdetails anti-VASP Antikörper

Spezifität: Recognizes both the 46 kDa (Ser157 dephospho-) and 50 kDa (Ser157 phospho-) form of human VASP.

Kreuzreaktivität: Human

Keine Kreuzreaktivität: Maus, Ratte (Rattus)

Kreuzreaktivität (Details): Does not cross-react with mouse or rat VASP.

Bestandteile: Contains Positive Control: Human platelet protein (500μg) (Prod. No. AG-35T-0001), supplied at 5mg/ml in SDS-stop solution (100mM NaCl, 73mM Tris/HCl pH 6.7, 10mM DTT, 8mM EDTA, 5% [v/v] glycerol, 2% [w/v] SDS, 10μg/ml bromophenol blue sodium salt). Use 5μl (25μg) per lane.

Immunogen: Recombinant human VASP fused to a His-tag.

Anwendungsinformationen

Applikationshinweise: Use 5 μL (25 μg) per lane.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Crude serum containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Haltbarkeit: 12 months

Details zu VASP

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Andere Bezeichnung: VASP

Hintergrund: VASP (vasodilator-stimulated phosphoprotein) is a proline-rich protein substrate of cAMP- and cGMP-dependent protein kinases. VASP is an actin-associated protein involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping proteins. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments and it plays a role in actin-based mobility of Listeria monocytogenes in host cells. It regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. VASP phosphorylation is used to monitor the effect of so-called antiplatelet drugs that reduce platelet reactivity and are used to prevent stent thrombosis, strokes and heart attacks in patients. Phosphorylation of VASP at Ser157 causes a mobility shift in SDS gel electrophoresis from 46 to 50 kDa, which has been used as a convenient marker to monitor cyclic nucleotide-dependent protein kinase activity.

UniProt: P50552

Pathways: T-Zell Rezeptor Signalweg, Regulation of Actin Filament Polymerization, Tube Formation