Poliovirus Receptor Antikörper (Biotin)

Produktnummer ABIN2743906

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Poliovirus Receptor in ELISA und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Poliovirus Receptor Antikörper (Biotin) (ABIN2743906)

Target: Poliovirus Receptor (PVR)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Poliovirus Receptor Antikörper ist konjugiert mit Biotin

Applikation: ELISA, Flow Cytometry (FACS)

Klon: ANC2B2

Produktdetails anti-Poliovirus Receptor Antikörper (Biotin)

Spezifität: Antibody ANC2B2 binds to cell surface CD155 on U-937 cells, and binds to recombinant CD155 in EIA.

Aufreinigung: Protein A purified

Immunogen: Recombinant Human CD155.

Type of Immunogen: Recombinant protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Flo

Usage: Ficoll prepared human peripheral blood mononuclear cells (PBMC) were stimulated overnight in culture with 5 μg/mL PHA. Five x 105 cultured human U-937 cells per tube were washed and pre incubated 5 minutes with 20 μL of 300 ^g/mL human IgG (to reduce non specific binding) after which they were incubated 45 minutes on ice with 80 μL of anti-human CD155/Biotin at concentration of 10 ^g/mL. They were then washed twice and incubated with 2o reagent Streptavidin/R-PE after which they were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.8 log10 fluorescent units when compared to an Isotype control Mouse IgG1/Biotin. Binding was blocked when reagent was pre incubated with a 10-fold excess of recombinant CD155-muIg. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C, avoid freeze-thaw cycles.

Details zu Poliovirus Receptor

Target: Poliovirus Receptor (PVR)

Andere Bezeichnung: PVR / CD155

Hintergrund: Name/Gene ID: PVR

Synonyms: PVR, CD155, Hpvr, HVED, Nectin-like 5, Nectin-like protein 5, Poliovirus receptors beta, Poliovirus receptor, PVS, Necl-5, CD155 antigen, NECL5, TAGE4

Gen-ID: 5817

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Cancer Immune Checkpoints, SARS-CoV-2 Protein Interaktom