NGFR Antikörper (Biotin)

Produktnummer ABIN2743244

Produktdetails anti-NGFR Antikörper (Biotin)

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NGFR Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS), ELISA

Spezifität: Antibody ANC271/3D7 binds to recombinant CD271-muIg in EIA, and to endogenous CD271 on cell surface of human Reh cells.

Aufreinigung: Protein A purified

Immunogen: Recombinant soluble human CD271.

Type of Immunogen: Recombinant protein

Klon: ANC271-3D7

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Flo

Usage: Five x 105 cultured human SW480 cells were harvested by EDTA, washed and pre incubated with 20 μL of 300 μg/mL human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 μL of anti-CD271/Biotin at a concentration of 10 μg/mL. Cells were then washed twice and incubated with secondary reagent Streptaviden/R-PE. They were then washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.17 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti-CD271 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C, avoid freeze-thaw cycles.

Details zu NGFR

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Andere Bezeichnung: NGFR / CD271 (NGFR Produkte)

Synonyme: ngfr antikoerper, wu:fb38c02 antikoerper, wu:fc10h01 antikoerper, CD271 antikoerper, Gp80-LNGFR antikoerper, TNFRSF16 antikoerper, p75(NTR) antikoerper, p75NTR antikoerper, LNGFR antikoerper, RNNGFRR antikoerper, Tnfrsf16 antikoerper, p75 antikoerper, NRH1b antikoerper, fullback antikoerper, nrh1 antikoerper, p75ntr antikoerper, tnfrsf16 antikoerper, p75NGFR antikoerper, nerve growth factor receptor antikoerper, neurotrophin receptor associated death domain antikoerper, nerve growth factor receptor S homeolog antikoerper, nerve growth factor receptor (TNFR superfamily, member 16) antikoerper, NGFR antikoerper, nradd antikoerper, Ngfr antikoerper, ngfr.S antikoerper

Hintergrund: Name/Gene ID: NGFR
Family: TNF Receptor

Synonyms: NGFR, CD271, CD271 antigen, gp80-LNGFR, Nerve growth factor receptor, p75 ICD, p75(NTR), p75NTR, TNFR superfamily, member 16, NGF receptor, TNFRSF16

Gen-ID: 4804

Pathways: NF-kappaB Signalweg, Neurotrophin Signalübertragung, Carbohydrate Homeostasis, Growth Factor Binding