NGFR Antikörper (Biotin)

Produktnummer ABIN2743244

Der Maus Monoklonal Anti-NGFR-Antikörper wurde für FACS und ELISA validiert. Er ist geeignet, NGFR in Proben von Human zu detektieren.

Kurzübersicht für NGFR Antikörper (Biotin) (ABIN2743244)

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NGFR Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS), ELISA

Klon: ANC271-3D7

Produktdetails anti-NGFR Antikörper (Biotin)

Spezifität: Antibody ANC271/3D7 binds to recombinant CD271-muIg in EIA, and to endogenous CD271 on cell surface of human Reh cells.

Aufreinigung: Protein A purified

Immunogen: Recombinant soluble human CD271.

Type of Immunogen: Recombinant protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Flo

Usage: Five x 105 cultured human SW480 cells were harvested by EDTA, washed and pre incubated with 20 μL of 300 μg/mL human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 μL of anti-CD271/Biotin at a concentration of 10 μg/mL. Cells were then washed twice and incubated with secondary reagent Streptaviden/R-PE. They were then washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.17 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti-CD271 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C, avoid freeze-thaw cycles.

Details zu NGFR

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Andere Bezeichnung: NGFR / CD271

Hintergrund: Name/Gene ID: NGFR
Family: TNF Receptor

Synonyms: NGFR, CD271, CD271 antigen, gp80-LNGFR, Nerve growth factor receptor, p75 ICD, p75(NTR), p75NTR, TNFR superfamily, member 16, NGF receptor, TNFRSF16

Gen-ID: 4804

Pathways: NF-kappaB Signalweg, Neurotrophin Signalübertragung, Carbohydrate Homeostasis, Growth Factor Binding