HSP27 Antikörper

Produktnummer ABIN2741544

Der Maus-Monoklonal Anti-HSP27-Antikörper wurde für WB, IHC, ELISA, IF, FACS, IP und IHC (p) validiert. Er ist geeignet zum Nachweis von HSP27 in Proben aus Human, Maus, Ratte, Schaf, Schimpanse und Huhn. }

Kurzübersicht für HSP27 Antikörper (ABIN2741544)

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reaktivität: Human, Maus, Ratte, Schaf, Schimpanse, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HSP27 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: G3-1

Produktdetails anti-HSP27 Antikörper

Spezifität: Human HSPB1 / HSP27

Aufreinigung: Protein G purified

Immunogen: Partially purified human HSP27 (earlier called 24K) from breast cancer MCF-7 cells.

Type of Immunogen: Cells

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Flo (0.5 - 1 μg/10 x10⁶), IF (0.5 - 1 μg/mL), IHC, IHC-P (0.5 - 1 μg/mL), IP, WB (0.25 - 0.5 μg/mL)

Usage: This antibody recognizes a 24-27  kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). HSP27 was recently found to be identical to the estrogen-induced [Character 93]p29[Character 94] and [Character 93]24K[Character 94] protein. About 50 % of breast carcinomas are positive for HSP27, especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells. ELISA: Order BSA-free format for coating.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 mg/mL BSA, 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2°C to 8°C.

Details zu HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Andere Bezeichnung: HSPB1 / HSP27

Hintergrund: Name/Gene ID: HSPB1

Synonyms: HSPB1, 28 kDa heat shock protein, Heat shock 27 kDa protein, Heat shock 27kD protein 1, Heat shock 27  kDa protein 1, HSP27, HSP28, HSP 27, Hsp25, SRP27, Stress-responsive protein 27, CMT2F, Heat shock 27 kd protein, Heat shock protein beta-1, HMN2B, HS.76067

Gen-ID: 3315

Pathways: MAPK Signalweg, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling