BTLA Antikörper (APC)

Produktnummer ABIN2738632

Dieser Anti-BTLA Antikörper ist ein Maus Monoklonal Antikörper zur Detektion von BTLA in FACS und ELISA. Geeignet für Human.

Kurzübersicht für BTLA Antikörper (APC) (ABIN2738632)

Target: BTLA (B and T Lymphocyte Associated (BTLA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BTLA Antikörper ist konjugiert mit APC

Applikation: Flow Cytometry (FACS), ELISA

Klon: ANC6E9

Produktdetails anti-BTLA Antikörper (APC)

Spezifität: Antibody from clone ANC6E9 binds to CD272 on the cell surface of human PBMC in Flow cytometry, and to recombinant CD272-muIg in EIA.

Aufreinigung: Protein A purified

Immunogen: Recombinant extracellular human CD272.

Type of Immunogen: Recombinant protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Flo

Usage: Five x 105 human peripheral blood mononuclear cells (PBMC) per tube were washed and incubated 45 minutes on ice with 80 μL of anti-CD272/APC at a 1:50 dilution (10 μg/mL). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.89 log10 fluorescent units when compared to Mouse IgG1/APC Isotype Control. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti-CD272 antibody. ELISA: Use for capture. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 500 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 15 % glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C. Do not freeze. Protect from light.

Details zu BTLA

Target: BTLA (B and T Lymphocyte Associated (BTLA))

Andere Bezeichnung: BTLA / CD272

Hintergrund: Name/Gene ID: BTLA

Synonyms: BTLA, BTLA1, B and T lymphocyte associated, CD272, B and T lymphocyte attenuator, B- and T-lymphocyte attenuator, CD272 antigen

Gen-ID: 151888

Pathways: Cancer Immune Checkpoints