POLA1 Antikörper

Produktnummer ABIN272161

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch POLA1 in WB. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für POLA1 Antikörper (ABIN272161)

Target: POLA1 (DNA Polymerase alpha (POLA1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POLA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-POLA1 Antikörper

Spezifität: This antibody detects endogenous levels of DNA pol α protein. (region surrounding Lys111)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: DO NOT FREEZE!

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu POLA1

Target: POLA1 (DNA Polymerase alpha (POLA1))

Andere Bezeichnung: POLA1

Hintergrund: DNA replication, recombination and repair, all of which are necessary for genomic stability, require the presence of exonucleases. In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair they function to excise damaged DNA fragments and correct recombinational mismatches. These exonucleases include the family of DNA polymerases. DNA pol α, β, δ and ε are involved in DNA replication and repair. DNA pol δ and DNA pol ε are multisubunit enzymes, with DNA pol δ consisting of two subunits: p125, which interacts with the sliding DNA clamp protein PCNA, and p50. The nuclear-encoded DNA pol γ is the only DNA polymerase required for the replication of the mitochondrial DNA. DNA pol ζ is ubiquitously expressed in various tissues and mediates the cellular mechanism of damage-induced mutagenesis. DNA pol θ is a DNA polymerase-helicase that binds ATP and is involved in the repair of interstrand crosslinks.Synonyms: DNA polymerase alpha catalytic subunit, DNA polymerase alpha catalytic subunit p180

Molekulargewicht: approx. 165 kDa

Gen-ID: 5422

NCBI Accession: NP_058633

UniProt: P09884

Pathways: SARS-CoV-2 Protein Interaktom