TOP3B Antikörper

Produktnummer ABIN272153

Der Kaninchen Polyklonal anti-TOP3B Antikörper wird verwendet zum Nachweis von TOP3B in Proben von Human und Maus. Er wurde validiert für WB und IHC (p).

Kurzübersicht für TOP3B Antikörper (ABIN272153)

Target: TOP3B (Topoisomerase (DNA) III beta (TOP3B))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TOP3B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-TOP3B Antikörper

Spezifität: This antibody detects endogenous levels of Topo IIIbeta-1 protein. (region surrounding Gln84)

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: DO NOT FREEZE!

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu TOP3B

Target: TOP3B (Topoisomerase (DNA) III beta (TOP3B))

Andere Bezeichnung: TOP3B

Hintergrund: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA by transiently breaking and rejoining DNA strands. Although DNA topoisomerase I and DNA topoisomerase II relax both positive and negative supercoils, DNA topoisomerase III relaxes only negative supercoils. DNA topoisomerase III alpha exists as a long and a short isoform, which are produced by alternative splicing. DNA topoisomerase III alpha, which localizes to the nucleolus, is constitutively expressed and remains at high levels throughout the cell cycle in HL-60 cells. DNA topoisomerase III beta exists as three isoforms, namely beta1, beta2 and beta3, also produced by alternative splicing. DNA topoisomerase III beta1 is expressed in testes, heart and skeletal muscle, whereas beta2 is expressed in thymus, kidney and pancreas.Synonyms: DNA topoisomerase 3-beta-1, DNA topoisomerase III beta-1, TOP3B1

Molekulargewicht: approx. 96 kDa

Gen-ID: 8940

NCBI Accession: NP_003926

UniProt: O95985