WASF1 Antikörper

Produktnummer ABIN272093

Der Kaninchen Polyklonal anti-WASF1 Antikörper wird verwendet zum Nachweis von WASF1 in Proben von Human und Maus. Er wurde validiert für WB, IF und IHC (p).

Kurzübersicht für WASF1 Antikörper (ABIN272093)

Target: WASF1 (WAS Protein Family, Member 1 (WASF1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser WASF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-WASF1 Antikörper

Spezifität: This antibody detects endogenous levels of WAVE1 protein. (region surrounding Ile119)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 1000approx. 1: 5000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: DO NOT FREEZE!

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu WASF1

Target: WASF1 (WAS Protein Family, Member 1 (WASF1))

Andere Bezeichnung: WASF1 / WAVE1

Hintergrund: WASP (for Wiskott-Aldrich syndrome protein) and N-WASP are downstream effectors of Cdc42 that are implicated in actin polymerization and cytoskeletal organization. The WASP family also includes VASP (vasodilator-stimulated phosphoprotein) and Mena (for mammalian enabled protein), which accumulate at focal adhesions and are also involved in the regulation of the actin cytoskeleton. The WAVE proteins are related to the WASP family proteins and are likewise involved in mediating actin reorganization downstream of the Rho family of small GTPases. The two protein homologs WAVE1 and WAVE2 specifically regulate membrane ruffling by inducing the formation of actin filament clusters in response to GTP binding and activating Rac. The WAVE proteins mediate this actin polymerization by cooperating with the Arp2/3 complex, a nucleation core, and thereby promoting the formation of actin filaments. WAVE1, which is also designated SCAR (for suppressor of cAR), is expressed primarily in the brain, while WAVE2 is widely expressed with the expression highest in peripheral blood leukocytes.Synonyms: KIAA0269, SCAR1, Verprolin homology domain-containing protein 1, WASP family protein member 1, WAVE-1, Wiskott-Aldrich syndrome protein family member 1

Molekulargewicht: approx. 65 kDa

Gen-ID: 8936

NCBI Accession: NP_001020105

UniProt: Q92558

Pathways: RTK Signalweg, Regulation of Actin Filament Polymerization