PKC zeta Antikörper

Produktnummer ABIN272064

Der Kaninchen Polyklonal anti-PKC zeta Antikörper (ABIN272064) detektiert spezifisch PKC zeta in WB, IF und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für PKC zeta Antikörper (ABIN272064)

Target: PKC zeta (PRKCZ) (Protein Kinase C, zeta (PRKCZ))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PKC zeta Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PKC zeta Antikörper

Spezifität: This antibody detects endogenous levels of PKC zeta protein. (region surrounding Pro556)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 1000approx. 1: 5000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: DO NOT FREEZE!

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu PKC zeta

Target: PKC zeta (PRKCZ) (Protein Kinase C, zeta (PRKCZ))

Andere Bezeichnung: PRKCZ

Hintergrund: Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (α, βI, Βii and γ) and novel (n) PKC isoforms (δ, ε , ζ, η and θ). Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC δ and ε are independent of Ca++. On the other hand, nPKC δ and ε, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities.Synonyms: PKC zeta, PKC-zeta, PKC2, Protein kinase C zeta type, nPKC-zeta

Molekulargewicht: approx. 67, 78 kDa

Gen-ID: 5590

NCBI Accession: NP_001028753

UniProt: Q05513

Pathways: NF-kappaB Signalweg, RTK Signalweg, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling