LGR5 Antikörper (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5) (N-Term)

Details for Product anti-LGR5 Antibody No. ABIN2689804, Anbieter: Anmelden zum Anzeigen
Antigen
  • lgr5-a
  • lgr5a
  • FEX
  • Gpr49
  • GPR49
  • GPR67
  • GRP49
  • HG38
  • leucine-rich repeat containing G protein-coupled receptor 5 L homeolog
  • leucine rich repeat containing G protein coupled receptor 5
  • leucine rich repeat containing G protein-coupled receptor 5
  • lgr5.L
  • Lgr5
  • LGR5
Epitop
N-Term
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5
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Reaktivität
Human
188
69
12
9
1
1
Wirt
Ratte
122
65
37
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser LGR5 Antikörper ist unkonjugiert
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5
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1
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Applikation
BioImaging (BI), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
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Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Marke BD Pharmingen™
Immunogen Human LGR5 DNA
Klon 8F2
Isotyp IgG2b, kappa
Kreuzreaktivität (Details) Mouse (Lack of Reactivity Confirmed in Development)
Produktmerkmale Lgr5 (leucine-rich-repeat-containing G-protein-coupled receptor 5) is a seven transmembrane-domain receptor that is a target gene for Wnt and marks stem cells in the small intestine, colon, stomach, and hair follicle. Lgr5 was initially identified as a potential stem cell marker due to restricted expression of Lgr5 in the intestinal crypt and labeling of rapidly cycling cells of the colon and intestine. Using both lineage tracing and organoid culture experiments, Lgr5 positive cells are capable of generating all types of the small intestine epithelium hence indicating that Lgr5 marks stem cells of the small intestine and colon. R-spondin growth factors, which are secreted agonists of the Wnt pathway, bind Lgr5. The binding of R-spondins to Lgr5 leads to recruitment of the Frizzled/LRP Wnt receptor complex, which binds to Wnt ligands and leads to downstream Wnt signaling. Lgr5 is up-regulated in colon and ovarian cancers and has been implicated in promotion of tumor growth and metastasis. The 8F2 monoclonal antibody recognizes an epitope in the N-terminal region of Human Lgr5. Western Blot analysis of human LGR5. Lysates from human epithelial kidney cells, HEK293T, that were transiently transfected with empty vector (lane 1) or human LGR5 (lane 2) were probed with Purified Rat anti-human LGR5 monoclonal antibody (

BD Pharmingen™ Purified Rat anti-Human Lgr5 (N-Terminal) - Purified - Clone 8F2 - Isotype Rat IgG2b, κ - Reactivity Hu, Ms - 0.1 mg
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Andere Bezeichnung Lgr5 (LGR5 Antibody Abstract)
Hintergrund Synonyms: GPR49, GPR67, HG38
Molekulargewicht ~110 kDa
Applikationshinweise Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen (Not Recommended)
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store undiluted at 4°C.
Produkt verwendet in: Yui, Nakamura, Sato, Nemoto, Mizutani, Zheng, Ichinose, Nagaishi, Okamoto, Tsuchiya, Clevers, Watanabe: "Functional engraftment of colon epithelium expanded in vitro from a single adult Lgr5⁺ stem cell." in: Nature medicine, Vol. 18, Issue 4, pp. 618-23, 2012 (PubMed).

Carmon, Gong, Lin, Thomas, Liu: "R-spondins function as ligands of the orphan receptors LGR4 and LGR5 to regulate Wnt/beta-catenin signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 28, pp. 11452-7, 2011 (PubMed).

Merlos-Suárez, Barriga, Jung, Iglesias, Céspedes, Rossell, Sevillano, Hernando-Momblona, da Silva-Diz, Muñoz, Clevers, Sancho, Mangues, Batlle: "The intestinal stem cell signature identifies colorectal cancer stem cells and predicts disease relapse." in: Cell stem cell, Vol. 8, Issue 5, pp. 511-24, 2011 (PubMed).

de Lau, Barker, Low, Koo, Li, Teunissen, Kujala, Haegebarth, Peters, van de Wetering, Stange, van Es, Guardavaccaro, Schasfoort, Mohri, Nishimori, Mohammed, Heck, Clevers: "Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling." in: Nature, Vol. 476, Issue 7360, pp. 293-7, 2011 (PubMed).

Barker, Huch, Kujala, van de Wetering, Snippert, van Es, Sato, Stange, Begthel, van den Born, Danenberg, van den Brink, Korving, Abo, Peters, Wright, Poulsom, Clevers: "Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro." in: Cell stem cell, Vol. 6, Issue 1, pp. 25-36, 2010 (PubMed).

Jaks, Barker, Kasper, van Es, Snippert, Clevers, Toftgård: "Lgr5 marks cycling, yet long-lived, hair follicle stem cells." in: Nature genetics, Vol. 40, Issue 11, pp. 1291-9, 2008 (PubMed).

Barker, van Es, Kuipers, Kujala, van den Born, Cozijnsen, Haegebarth, Korving, Begthel, Peters, Clevers: "Identification of stem cells in small intestine and colon by marker gene Lgr5." in: Nature, Vol. 449, Issue 7165, pp. 1003-7, 2007 (PubMed).

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