IL-8 Antikörper

Produktnummer ABIN2689787

Dieses Maus Monoklonal-Antikörper erkennt spezifisch IL-8 in ELISA, FACS und Inhibition. Er zeigt eine Reaktivität gegenüber Human und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für IL-8 Antikörper (ABIN2689787)

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IL-8 Antikörper ist unkonjugiert

Applikation: ELISA, Flow Cytometry (FACS), Blocking Antibody (Inhibition)

Klon: G265

Produktdetails anti-IL-8 Antikörper

Marke: BD Pharmingen™

Produktmerkmale: The G265-8 antibody reacts with both the 72 and 77 amino acid forms of human interleukin-8 (IL-8). The immunogen used to produce the G265-8 hybridoma was E. coli-expressed recombinant human IL-8. Expression of IL-8 by stimulated CD14+ human monocytes. Human PBMC were stimulated for 6 hours with LPS (10 ng/mL final concentration) in the presence of GolgiStop™ (2 μM final concentration, Cat. No. 554724). The PBMC were harvested, stained with FITC-mouse anti-human CD14 antibody (FITC-M5E2, Cat. No. 555397), fixed, permeabilized, and subsequently stained with 0.25 μg of PE-mouse anti-human IL-8 antibody (PE-G265-8, Cat. No. 554720) following the BD Pharmingen staining protocol (left panel). The data reflect gating on monocytes, based on forward and side scattered light signals. To demonstrate specificity of staining, the binding of PE-G265-8 was blocked by the preincubation of the conjugated antibody with recombinant human IL-8 (0.25 μg, Cat. No. 554609, center panel), and by preincubation of the fixed/permeabilized cells with unlabelled G265-8 antibody (2.5 μg, Cat. No. 554717, right panel) prior to staining with the PE-G265-8 antibody. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking (center panel) and unlabelled antibody (right panel) blocking specificity controls.

BD Pharmingen™ Purified Mouse Anti-Human IL-8 - Purified - Clone G265-8 - Isotype Mouse IgG2b - Reactivity Hu - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Recombinant Human IL-8

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-IL-8 Antikörper (ABIN2689787)

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Matsushima, Oppenheim: "Interleukin 8 and MCAF: novel inflammatory cytokines inducible by IL 1 and TNF." in: Cytokine, Vol. 1, Issue 1, pp. 2-13, (1991) (PubMed).

Details zu IL-8

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Andere Bezeichnung: IL-8

Pathways: TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagie