IL-10 Antikörper

Produktnummer ABIN2689722

Der Ratte Monoklonal Anti-IL-10-Antikörper wurde für ELISA, Neut und Inhibition validiert. Er ist geeignet, IL-10 in Proben von Human und Virus zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für IL-10 Antikörper (ABIN2689722)

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Reaktivität: Human, Virus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser IL-10 Antikörper ist unkonjugiert

Applikation: ELISA, Neutralization (Neut), Blocking Antibody (Inhibition)

Klon: JES3

Produktdetails anti-IL-10 Antikörper

Marke: BD Pharmingen™

Produktmerkmale: The JES3-19F1 antibody reacts with human (IL-10). The immunogen used to generate the JES3-19F1 hybridoma was recombinant human IL-10 expressed in COS cells. This is a neutralizing antibody. This antibody also reacts with viral IL-10. Expression of IL-10 by stimulated CD14+ human monocytes. Human PBMC were stimulated for 24 hours with LPS (1.0 μg/mL) in the presence of GolgiStop™ (Cat. No. 554724, 2 μM final concentration). The PBMC were harvested, stained with FITC-mouse anti-human CD14 antibody (FITC-M5E2, Cat. No. 555397), fixed, permeabilized, and subsequently stained with 0.25 μg of PE-rat anti-human IL-10 antibody (PE-JES3-19F1, Cat. No. 554706) following Pharmingen's staining protocol (see image, left panel). The data reflects gating on monocytes, based on forward and side scattered light signals. To demonstrate specificity of staining, the binding of the PE-JES3-19F1 antibody was blocked by the preincubation of the conjugated antibody with recombinant human IL-10 (0.5 mg, Cat. No. 554611, middle panel), and by preincubation of the fixed/permeabilized cells with unlabeled JES3-19F1 antibody (10 mg, Cat. No. 554704, right panel) prior to staining with the PE-JES3-19F1 antibody. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking (middle panel) and unlabeled antibody (right panel) blocking specificity controls.

BD Pharmingen™ Purified Rat Anti-Human IL-10 - Purified - Clone JES3-19F1 - Isotype Rat IgG2a - Reactivity Hu, Vir - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Recombinant Human IL-10

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-IL-10 Antikörper (ABIN2689722)

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Andersson, Christensen, Marker, Thomsen: "Changes in cell adhesion molecule expression on T cells associated with systemic virus infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 3, pp. 1237-45, (1994) (PubMed).

Details zu IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Andere Bezeichnung: IL-10

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints