CD3 Antikörper

Produktnummer ABIN2689168

Produktdetails anti-CD3 Antikörper

Target: CD3

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD3 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB), Cytotoxicity Test (CyTox)

Marke: BD Pharmingen™

Produktmerkmale: The G4.18 antibody reacts with the T-cell receptor-associated CD3 cell-surface antigen found on thymocytes, peripheral T lymphocytes, and dendritic epidermal T cells. It has been reported that CD3 expression is down-regulated within 24 hours in concanavalin A-stimulated rat T cells, and soluble mAb inhibits the allogeneic mixed-lymphocyte proliferative response and cell-mediated cytotoxicity to allogeneic target cells. In vivo treatment with G4.18 mAb prevents cardiac and skin allograft rejection, resulting in donor-specific tolerance. Pre-incubation of splenocytes with the alternate anti-rat CD3 monoclonal antibody, 1F4 (Cat. No. 556970), blocks staining with mAb G4.18.

BD Pharmingen™ Purified Mouse Anti-Rat CD3 - Purified - Clone G4.18 - Isotype Mouse IgG3, κ - Reactivity Rat - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: PHA-stimulated rat lymph node and spleen cells

Klon: G4-18

Isotyp: IgG3 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-CD3 Antikörper (ABIN2689168)

Morris, Komocsar: "Immunophenotyping analysis of peripheral blood, splenic, and thymic lymphocytes in male and female rats." in: Journal of pharmacological and toxicological methods, Vol. 37, Issue 1, pp. 37-46, (1997) (PubMed).

Strickland, Kees, Holt: "Regulation of T-cell activation in the lung: alveolar macrophages induce reversible T-cell anergy in vitro associated with inhibition of interleukin-2 receptor signal transduction." in: Immunology, Vol. 87, Issue 2, pp. 250-8, (1996) (PubMed).

Naper, Vaage, Lambracht, Løvik, Butcher, Wonigeit, Rolstad: "Alloreactive natural killer cells in the rat: complex genetics of major histocompatibility complex control." in: European journal of immunology, Vol. 25, Issue 5, pp. 1249-56, (1995) (PubMed).

Upham, Strickland, Bilyk, Robinson, Holt: "Alveolar macrophages from humans and rodents selectively inhibit T-cell proliferation but permit T-cell activation and cytokine secretion." in: Immunology, Vol. 84, Issue 1, pp. 142-7, (1995) (PubMed).

Nicolls, Aversa, Pearce, Spinelli, Berger, Gurley, Hall: "Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody." in: Transplantation, Vol. 55, Issue 3, pp. 459-68, (1993) (PubMed).

Details zu CD3

Target: CD3

Andere Bezeichnung: CD3 (CD3 Produkte)

Synonyme: CD3 antikoerper, AI504783 antikoerper, CD3epsilon antikoerper, T3e antikoerper, cd3-gamma antikoerper, cd3g antikoerper, cd3g-a antikoerper, cd3g/d antikoerper, cd3gd antikoerper, t3g antikoerper, CD3e molecule antikoerper, CD3d molecule antikoerper, CD3 antigen, epsilon polypeptide antikoerper, CD3g molecule S homeolog antikoerper, CD3E antikoerper, CD3D antikoerper, Cd3e antikoerper, cd3g.S antikoerper

Pathways: T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway