CD3 Antikörper

Produktnummer ABIN2689164

Es sind 3+ Publikationen für dieses Produkt verfügbar. Der Ratte Monoklonal anti-CD3 Antikörper wird verwendet zum Nachweis von CD3 in Proben von Maus. Er wurde validiert für FACS, IP, IHC (fro) und CyTox.

Kurzübersicht für CD3 Antikörper (ABIN2689164)

Target: CD3

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD3 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytotoxicity Test (CyTox)

Klon: 17A2

Produktdetails anti-CD3 Antikörper

Produktmerkmale: The 17A2 antibody reacts with the T-cell receptor-associated CD3 complex, which is expressed on many thymocytes and mature T lymphocytes. Plate-bound mAb 17A2 has been reported to induce IL-2 production by T cells in the absence of accessory cells. Binding of 17A2 antibody to T cells can be blocked by the anti-CD3e mAb 145-2C11, suggesting that 17A2 antibody recognizes an epitope of the CD3 epsilon chain. In vivo treatment with 17A2 mAb has been reported to partially deplete T lymphocytes and temporarily down-modulates CD3 expression on T cells. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. CD3 expression in spleen and thymus. BALB/c splenocytes were simultaneously stained with purified mAb 17A2 (bottom left panel) and PE-conjugated anti-mouse CD19 mAb 1D3 (top left & bottom left panels), followed by FITC-conjugated anti-rat IgG2b mAb G15-337 (Cat. No. 553884) (top left & bottom left panels). BALB/c thymocytes were also stained with 17A2 mAb (bottom right panel), followed by FITC-conjugated anti-rat IgG2b (top right & bottom right panels). Flow cytometry was performed on a BD FACSCan™ flow cytometry system.

BD Pharmingen™ Purified Rat Anti-Mouse CD3 Molecular Complex - Purified - Clone 17A2 - Isotype Rat IgG2b, κ - Reactivity Ms - 0.5 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: gammadelta TCR-positive T-T hybridoma D1

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-CD3 Antikörper (ABIN2689164)

Mysliwietz, Thierfelder et al.: "Antilymphocytic antibodies and marrow transplantation. XII. Suppression of graft-versus-host disease by T-cell-modulating and depleting antimouse CD3 antibody is most effective when preinjected in ..." in: Blood, Vol. 80, Issue 10, pp. 2661-7, (1992) (PubMed).

Wu, Antica, Johnson, Scollay, Shortman: "Developmental potential of the earliest precursor cells from the adult mouse thymus." in: The Journal of experimental medicine, Vol. 174, Issue 6, pp. 1617-27, (1992) (PubMed).

Miescher, Schreyer, MacDonald: "Production and characterization of a rat monoclonal antibody against the murine CD3 molecular complex." in: Immunology letters, Vol. 23, Issue 2, pp. 113-8, (1990) (PubMed).

Details zu CD3

Target: CD3

Andere Bezeichnung: CD3

Pathways: T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway