This antibody reacts with the 65-70 kDa common γ subunit (γc) shared by the IL-2, IL-4, IL-7, IL-9, and IL-15 receptors. The γc receptor is a glycoprotein expressed by most peripheral T and B lymphocytes, NK cells, monocytes, and granulocytes. The cytoplasmic domain of the γc chain plays an important role in cytokine-mediated signal transduction. By immunofluorescent staining and flow cytometric analysis, the TUGh4 antibody has been shown to specifically recognize human γc expressed by cell lines, including human γc gene-transfected cell lines, which are known to express the human γc chain.
BD Pharmingen™ Biotin Rat Anti-Human CD132 - Biotin - Clone TUGh4 - Isotype Rat IgG2b, κ - Reactivity Hu, Dog - 0.5 mg
Aufreinigung
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
IL2RG
Reaktivität: Human
WB
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Optimal working dilution should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
0.5 mg/mL
Buffer
Aqueous buffered solution containing protein stabilizer and ≤0.09 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Lagerung
4 °C
Informationen zur Lagerung
Store undiluted at 4°C.
Ishii, Takeshita, Kimura, Tada, Kondo, Nakamura, Sugamura: "Expression of the IL-2 receptor gamma chain on various populations in human peripheral blood." in: International immunology, Vol. 6, Issue 8, pp. 1273-7, (1995) (PubMed).
Guesdon, Ternynck, Avrameas: "The use of avidin-biotin interaction in immunoenzymatic techniques." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).