ITGAL Antikörper

Produktnummer ABIN2688903

Der Maus Monoklonal Anti-ITGAL-Antikörper wurde für FACS, IP, IHC (fro) und BR validiert. Er ist geeignet, ITGAL in Proben von Ratte zu detektieren. Es sind 11+ Publikationen verfügbar.

Kurzübersicht für ITGAL Antikörper (ABIN2688903)

Target: ITGAL (Integrin alpha-L (ITGAL))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ITGAL Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR)

Klon: WT-1

Produktdetails anti-ITGAL Antikörper

Marke: BD Pharmingen™

Produktmerkmale: The WT.5 antibody reacts with the asubunit of LFA-1 (αLβ2 integrin, CD11a/CD18), a heterodimeric surface glycoprotein which is found on the majority of leukocytes, but not on peritoneal macrophages or peritoneal mast cells. LFA-1 mediates a variety of heterotypic and homotypic intercellular adhesions through interaction with ICAM-1 (CD54) and ICAM-2 (CD102). WT.1 mAb recognizes both the activated and unactivated forms of LFA-1. It inhibits the binding of LFA-1 to ICAM-1 in several in vitro assays, including binding of Concanavalin A-stimulated lymphocytes (Con A blasts) to purified ICAM-1 and Mg2+-dependent aggregation of concanavalin A-stimulated blasts. It has also been reported to inhibit leukocyte infiltration in several in vivo models of inflammation. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Mouse Anti-Rat CD11a - Purified - Clone WT.1 - Isotype Mouse IgG2a, κ - Reactivity Rat - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: PHA-stimulated rat splenocytes and rat thymic lymphoma FTL-43

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-ITGAL Antikörper (ABIN2688903)

Ohta, Gotoh, Ohzato, Fukuzaki, Nishihara, Dono, Umeshita, Sakon, Yagita, Okumura, Tanaka, Kawashima, Miyasaka, Monden: "Direct antigen presentation through binding of donor intercellular adhesion molecule-1 to recipient lymphocyte function-associated antigen-1 molecules in xenograft rejection." in: Transplantation, Vol. 65, Issue 8, pp. 1094-100, (1998) (PubMed).

Andrews, Malcontenti-Wilson, OBrien: "Expression of adhesion molecules and leukocyte recruitment into gastric mucosa following ischemia-reperfusion." in: Digestive diseases and sciences, Vol. 42, Issue 2, pp. 326-32, (1997) (PubMed).

Watanabe, Arakawa, Fukuda, Higuchi, Kobayashi: "Role of neutrophils in a rat model of gastric ulcer recurrence caused by interleukin-1 beta." in: The American journal of pathology, Vol. 150, Issue 3, pp. 971-9, (1997) (PubMed).

Wada, Shikata, Makino, Morioka, Hirata, Ota, Tamatani, Miyasaka, Horiuchi, Noji, Nishikawa, Myokai, Taniguchi, Kanwar, Ota: "The critical role of intercellular adhesion molecule-1 in Masugi nephritis in rats." in: Nephron, Vol. 73, Issue 2, pp. 264-72, (1996) (PubMed).

Devine, Lightman, Greenwood: "Role of LFA-1, ICAM-1, VLA-4 and VCAM-1 in lymphocyte migration across retinal pigment epithelial monolayers in vitro." in: Immunology, Vol. 88, Issue 3, pp. 456-62, (1996) (PubMed).

Fox, Jewell, Whitacre: "Rat peritoneal mast cells present antigen to a PPD-specific T cell line." in: Cellular immunology, Vol. 158, Issue 1, pp. 253-64, (1994) (PubMed).

Springer: "Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm." in: Cell, Vol. 76, Issue 2, pp. 301-14, (1994) (PubMed).

Bañuls, Alvarez, Ferrero, Zapata, Ardavin: "Cell-surface marker analysis of rat thymic dendritic cells." in: Immunology, Vol. 79, Issue 2, pp. 298-304, (1993) (PubMed).

Yamazaki, Seko, Tamatani, Miyasaka, Yagita, Okumura, Nagai, Yazaki: "Expression of intercellular adhesion molecule-1 in rat heart with ischemia/reperfusion and limitation of infarct size by treatment with antibodies against cell adhesion molecules." in: The American journal of pathology, Vol. 143, Issue 2, pp. 410-8, (1993) (PubMed).

Tamatani, Kotani, Miyasaka: "Characterization of the rat leukocyte integrin, CD11/CD18, by the use of LFA-1 subunit-specific monoclonal antibodies." in: European journal of immunology, Vol. 21, Issue 3, pp. 627-33, (1991) (PubMed).

Larson, Springer: "Structure and function of leukocyte integrins." in: Immunological reviews, Vol. 114, pp. 181-217, (1990) (PubMed).

Details zu ITGAL

Target: ITGAL (Integrin alpha-L (ITGAL))

Andere Bezeichnung: CD11a

Hintergrund: Synonyms: Integrin αL chain, LFA-1 α chain

Pathways: Activated T Cell Proliferation, Integrin Complex