Cyclin E1 Antikörper (AA 307-410)

Produktnummer ABIN2682980

Dieses Anti-Cyclin E1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Cyclin E1 in ELISA und FACS. Geeignet für Human und Maus.

Kurzübersicht für Cyclin E1 Antikörper (AA 307-410) (ABIN2682980)

Target: Cyclin E1 (CCNE1)

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin E1 Antikörper ist unkonjugiert

Applikation: ELISA, Flow Cytometry (FACS)

Klon: 5F8C5

Produktdetails anti-Cyclin E1 Antikörper

Bindungsspezifität: AA 307-410

Verwendungszweck: CCNE1 Antibody

Aufreinigung: Purified antibody

Immunogen: Purified recombinant fragment of human CCNE1 (AA: 307-410) expressed in E. Coli.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

ELISA: 1/10000

FCM: 1/200 - 1/400

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Details zu Cyclin E1

Target: Cyclin E1 (CCNE1)

Andere Bezeichnung: CCNE1

Hintergrund: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available.

Molekulargewicht: 47 kDa

Gen-ID: 898

UniProt: P24864

Pathways: Zellzyklus, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases