EED Antikörper

Produktnummer ABIN2668749

Dieser Maus Monoklonal Antikörper detektiert spezifisch EED in WB, IHC, ChIP-seq und ChIP. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für EED Antikörper (ABIN2668749)

Target: EED (Embryonic Ectoderm Development (EED))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EED Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ChIP DNA-Sequencing (ChIP-seq), Chromatin Immunoprecipitation (ChIP)

Klon: 41D

Produktdetails anti-EED Antikörper

Verwendungszweck: EED antibody (mAb) (Clone 41D)

Aufreinigung: Protein A Chromatography

Immunogen: This EED antibody was raised against full-length recombinant human EED protein.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: ChIP: 4 µL per ChIP ChIP-Seq: 4 µL each IHC: 2 - 8 μg/mL dilution This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91135.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu EED

Target: EED (Embryonic Ectoderm Development (EED))

Andere Bezeichnung: EED

Hintergrund: EED (extraembryonic ectoderm) is a component of the Polycomb group (PcG) multiprotein complex PRC2. The PRC2 complex also contains EZH2 and SUZ12 and is recruited to Polycomb response elements and results in the methylation of histone H3 and lysine 27 and the silencing of gene expression. PRC2 initiates repression of the Hox genes during development and is involved in the maintenance of stem cell pluripotency. EED is a member of the superfamily of WD-40 repeat protein family and is required for PRC2-mediated H3K27 methylation. The PRC2 complex may also recruit platform DNMTs, thereby linking two systems of epigenetic repression.

Molekulargewicht: 57 kDa

Gen-ID: 8726

NCBI Accession: NP_003788