BMI1 Antikörper

Produktnummer ABIN2668620

Dieser Maus Monoklonal Antikörper detektiert spezifisch BMI1 in WB, IF und ICC. Es zeigt Reaktivität gegenüber Proben von Human und Maus.Es wurde in 1 Publikation zitiert.

Kurzübersicht für BMI1 Antikörper (ABIN2668620)

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BMI1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Klon: DC9

Produktdetails anti-BMI1 Antikörper

Verwendungszweck: BMI-1 antibody (mAb) (Clone DC9)

Aufreinigung: Protein G Chromatography

Immunogen: This BMI-1 antibody was raised against a recombinant protein corresponding to full-length mouse BMI-1.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: ICC/IF: 0.5 μg/mL dilution For ChIP-Seq analysis of BMI-1, we offer AbFlex BMI-1 Recombinant Antibody (rAb). For details, see Catalog No. 91195.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-BMI1 Antikörper (ABIN2668620)

Bracken, Kleine-Kohlbrecher, Dietrich, Pasini, Gargiulo, Beekman, Theilgaard-Mönch, Minucci, Porse, Marine, Hansen, Helin: "The Polycomb group proteins bind throughout the INK4A-ARF locus and are disassociated in senescent cells." in: Genes & development, Vol. 21, Issue 5, pp. 525-30, (2007) (PubMed).

Details zu BMI1

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Andere Bezeichnung: BMI-1

Hintergrund: BMI-1 is a member of the Polycomb PRC1 complex that is recruited to transcriptionally repressed genes subsequent to histone H3 lysine 27 methylation in order to maintain repression. In the PRC1 complex, BMI-1 is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2, resulting in the ubiquitylation of histone H2A at lysine 119. BMI-1 acts as an oncogene and cooperates with c-myc in the initiation of lymphoma. High levels of expression of BMI-1 are observed in metastatic melanoma, and BMI-1 has been implicated in several other cancers. BMI-1 is required for hematopoietic stem cell self renewal.

Molekulargewicht: 40 kDa

Gen-ID: 648

NCBI Accession: NP_031578

Pathways: Zellzyklus, Autophagie