Histone H2A Antikörper (H2AK9ac)

Produktnummer ABIN2668302

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Histone H2A in WB, IF und DB. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Histone H2A Antikörper (H2AK9ac) (ABIN2668302)

Target: Histone H2A

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone H2A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB)

Produktdetails anti-Histone H2A Antikörper

Bindungsspezifität: H2AK9ac

Verwendungszweck: Histone H2AK9ac antibody (pAb)

Aufreinigung: Unpurified

Immunogen: This Histone H2A acetyl Lys9 antibody was raised against a peptide including acetyl-lysine 9 of human histone H2A.

Anwendungsinformationen

Applikationshinweise: WB*: 1:2,000 - 1:5,000 dilution IF: 1:500 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu Histone H2A

Target: Histone H2A

Hintergrund: Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H2A and Histone H2B are acetylated in bulk chromatin by p300 and form acetylated Histone H2A/Histone H2B heterodimers. When DNA associates with intact core histone octamers that contain acetylated H2A/H2B dimers, the inhibition of transcriptional initiation significantly decreases, indicating that acetylation of their lysine residues may mediate transcription.

Molekulargewicht: 14 kDa

Gen-ID: 92815

NCBI Accession: NP_003508