Histone H2A Antikörper (pThr120)

Produktnummer ABIN6971791

Produktdetails anti-Histone H2A Antikörper

Target: Histone H2A

Bindungsspezifität: pThr120

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone H2A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB)

Produktmerkmale: Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Phosphorylation of histones occurs at multiple sites during mitosis. H2A Thr120 phosphorylation is observed on chromatin during both mitosis and meiosis. Thr120 phosphorylation is inversely correlated with ubiquitylation of H2A Lys119 in meiotic mouse spermatocytes. In Drosophila, loss of H2A Thr120 phosphorylation is associated with a failure to disassemble the synaptonemal complex, impaired loading of condensin and female infertility. It is possible that H2A Thr120 phosphorylation is involved in the regulation of chromatin structure. Histone H2AT120ph antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot, Immunofluorescence and Western blot, it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.

Aufreinigung: Protein A Chromatography

Immunogen: This Histone H2A phospho Thr120 antibody was raised against a peptide containing phospho Thr120 of human histone H2A.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Details zu Histone H2A

Target: Histone H2A

Abstract: Histone H2A Produkte

Synonyme: 5499 antikoerper, CG5499 antikoerper, Dmel\\CG5499 antikoerper, H2A antikoerper, H2A.F/Z antikoerper, H2A.X antikoerper, H2A.Z antikoerper, H2AV antikoerper, H2AV_DROM antikoerper, H2AX antikoerper, H2AZ antikoerper, H2Av antikoerper, H2AvD antikoerper, H2a.V antikoerper, H2av antikoerper, H2avD antikoerper, HIS antikoerper, HIS2AV antikoerper, HIS2AVD antikoerper, His antikoerper, His2 antikoerper, His2AV antikoerper, His2AvD antikoerper, HisH2Av antikoerper, Hist antikoerper, Hist2av antikoerper, gamma-H2Av antikoerper, gamma-HIS2AV antikoerper, gamma-His2Av antikoerper, gammaH2Av antikoerper, h2AvD antikoerper, his antikoerper, l(3)05146 antikoerper, l(3)810 antikoerper, l(3)97Dd antikoerper, l(3)L1602 antikoerper, DDBDRAFT_0205974 antikoerper, DDBDRAFT_0216271 antikoerper, DDB_0205974 antikoerper, DDB_0216271 antikoerper, RGD1564767 antikoerper, H2a-221 antikoerper, H2A-III antikoerper, HIST1H2AA antikoerper, Histone H2A variant antikoerper, putative histone H2A antikoerper, histone H2A antikoerper, histone cluster 1 H2A family member F antikoerper, histone H2A-like antikoerper, histone cluster 1, H2af antikoerper, histone2A1 antikoerper, histone cluster 1, H2A, III antikoerper, histone cluster 1, H2ac antikoerper, His2Av antikoerper, LMJF_17_0280 antikoerper, H2AX antikoerper, Hist1h2af antikoerper, LOC100425378 antikoerper, his2a1 antikoerper, HIST1H2A3 antikoerper, HIST1H2AC antikoerper, h2a-A antikoerper

Molekulargewicht: 14 kDa

NCBI Accession: NP_003508