POLR2B Antikörper (N-Term)

Produktnummer ABIN2668294

Der Kaninchen Polyklonal anti-POLR2B Antikörper wird verwendet zum Nachweis von POLR2B in Proben von Human. Er wurde validiert für WB und IP.

Kurzübersicht für POLR2B Antikörper (N-Term) (ABIN2668294)

Target: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POLR2B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-POLR2B Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: POLR2B antibody (pAb)

Aufreinigung: Affinity Purified

Immunogen: This antibody was raised against a peptide within the N-terminal region of human POLR2B.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IP: 5 - 10 µL per IP WB*: 1:500 - 1:1,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu POLR2B

Target: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Andere Bezeichnung: POLR2B

Hintergrund: POLR2B (polymerase (RNA) II (DNA directed) polypeptide B, 140 kDa) DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity).

Molekulargewicht: 135 kDa

Gen-ID: 5431

NCBI Accession: NP_000929

Pathways: Regulatorische RNA Pathways, DNA Reparatur