PLK1 Antikörper (pThr210)

Produktnummer ABIN2665331

Dieses Anti-PLK1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von PLK1 in BCA. Geeignet für Human.

Kurzübersicht für PLK1 Antikörper (pThr210) (ABIN2665331)

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PLK1 Antikörper ist unkonjugiert

Applikation: Biochemical Assay (BCA)

Klon: 2A3

Produktdetails anti-PLK1 Antikörper

Bindungsspezifität: pThr210

Aufreinigung: The antibody was purified by affinity chromatography.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide at 0.5 mg/mL.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: The antibody solution should be stored undiluted between 2°C and 8°C.

Details zu PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Andere Bezeichnung: PLK-1

Hintergrund: PLK-1 (polo-like kinase 1) is a member of te serine/threonine protein kinase family, cdc5/polo subfamily. Highly homologous to polo-like kinase (Drosophila), PLK-1 contains two polo box domains with a predicted molecular weight of 68 kD. This nuclear protein is highly expressed in placenta and colon and has been shown to regulate cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase. PLK-1 may also be required for cell division, depletion of PLK-1 results in apoptosis. PLK-1 is upregulated by growth stimulating agents and is regulated by cell cycle position (highest in G2/M phase, declining to nearly undetectable levels after mitosis and throughout G1). PLK-1 is modified by phosphorylation (Thr210 is the major phosphorylation site in activated PLK-1 from mitotic cells) and has been shown to interact with nuclear distribution gene C. The 2A3 antibody recognizes human phosphorylated PLK-1 (Thr210) and has been shown to be useful for Western blotting. To increase specificity, it is recommended that the 2A3 antibody be used for Western blotting after immunoprecipitation with the pan-specific PLK-1 3F8 antibody.

Pathways: Zellzyklus, M Phase