Cullin 1 Antikörper
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- Target Alle Cullin 1 (CUL1) Antikörper anzeigen
- Cullin 1 (CUL1)
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Reaktivität
- Human, Maus
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser Cullin 1 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Spezifität
- This antibody detects endogenous levels of Cullin-1 protein (region surrounding Tyr761).
- Aufreinigung
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
- Reinheit
- > 95 % pure by SDS-PAGE
- Top Product
- Discover our top product CUL1 Primärantikörper
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- Applikationshinweise
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ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- Cullin 1 (CUL1)
- Andere Bezeichnung
- Cullin 1 (CUL1 Produkte)
- Synonyme
- LOC100282292 antikoerper, Cullin-1 antikoerper, Cul1 antikoerper, zgc:55747 antikoerper, zgc:77500 antikoerper, wu:fc13a10 antikoerper, cullin-1 antikoerper, ATCUL1 antikoerper, AUXIN RESISTANT 6 antikoerper, AXR6 antikoerper, T10P11.26 antikoerper, T10P11_26 antikoerper, cullin 1 antikoerper, cul1 antikoerper, wu:fi43h07 antikoerper, wu:fj43a08 antikoerper, zgc:111899 antikoerper, zgc:55483 antikoerper, Cul-1 antikoerper, cullin 1 antikoerper, cullin-1 antikoerper, Cullin 1 antikoerper, Cullin-1 antikoerper, cullin 1a antikoerper, cullin 1 S homeolog antikoerper, cullin 1b antikoerper, CUL1 antikoerper, LOC100282292 antikoerper, cul1 antikoerper, ATEG_00491 antikoerper, EDI_072700 antikoerper, EDI_264400 antikoerper, SJAG_02591 antikoerper, BDBG_03785 antikoerper, Cul1 antikoerper, cul1a antikoerper, cul1.S antikoerper, cul-1 antikoerper, cul1b antikoerper
- Hintergrund
- CUL-1, which is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1-mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3, facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase.Synonyms: CUL-1, CUL1, Cullin-1
- Molekulargewicht
- approx. 90 kDa
- Gen-ID
- 8454
- NCBI Accession
- NP_003583
- UniProt
- Q13616
- Pathways
- Zellzyklus, Hedgehog Signalweg, Mitotic G1-G1/S Phases, Regulation of Cell Size
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