Cyclin E1 Antikörper

Produktnummer ABIN264331

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Cyclin E1 in WB und IP. Er zeigt eine Reaktivität gegenüber Human und Affe.

Kurzübersicht für Cyclin E1 Antikörper (ABIN264331)

Target: Cyclin E1 (CCNE1)

Reaktivität: Human, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin E1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Klon: 168

Produktdetails anti-Cyclin E1 Antikörper

Produktmerkmale: Synonyms: Cyclin-E1, CCNE1, CCNE

Aufreinigung: Purified

Immunogen: Recombinant full-length human cyclin E protein.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Western Blot: A 50 kDa band corresponding to human cyclin E protein is detected. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.08 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Haltbarkeit: 12 months

Details zu Cyclin E1

Target: Cyclin E1 (CCNE1)

Andere Bezeichnung: Cyclin E1

Hintergrund: Human cyclin E was originally identified by complementation of a triple cln deletion in Saccharomyces cerevisiae. Following the identification of human cyclin E protein, researchers found that breast cancers and some other solid tumors exhibited both quantitative and qualitative alterations in cyclin E protein production. In particular, the alterations in cyclin E expression in breast cancer correlated with increasing stage and grade of the tumor. These results suggested that cyclin E might be useful as a prognostic marker. More recently, Geng et al generated a mouse strain in which the coding sequences for cyclin D1 was deleted and replaced with those of human cyclin E. Replacement of cyclin D1 with cyclin E rescued all phenotypic manifestations seen in the cyclin D1 deficient mice and restored normal development to cyclin D1-dependent tissues. Based on the results of these studies, it appears that cyclin E is the major downstream target of cyclin D1. In addition to functioning downstream of cyclin D1, recent studies with Xenopus egg extracts demonstrated that cyclin E localizes to the centrosome. In these studies CDK2-cyclin E activity was shown to be required for centrosome duplication during S phase. Taken together, these data suggest a mechanism that coordinates centrosome reproduction with cycles of DNA synthesis and mitosis.Synonyms: CCNE, CCNE1, Cyclin-E1

Gen-ID: 898

UniProt: P24864

Pathways: Zellzyklus, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases