Nitrotyrosine Antikörper

Produktnummer ABIN263971

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Nitrotyrosine in WB, IP, IHC (fro) und EIA. Er zeigt eine Reaktivität gegenüber .

Kurzübersicht für Nitrotyrosine Antikörper (ABIN263971)

Target: Nitrotyrosine

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Nitrotyrosine Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Klon: 39B6

Produktdetails anti-Nitrotyrosine Antikörper

Produktmerkmale: Synonyms: NO-Tyrosine, Nitro-Tyrosine

Aufreinigung: Affinity Chromatography on Protein G.

Immunogen: 3-(4-Hydroxy-3-Nitrophenyl Acetamido) Propionic Acid-BSA conjugate

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: ELISA (1,6). Immunoprecipitation. Western blot (1): 0.7 μg/mL was sufficient for detection of 5 μg SN-1 treated BSA using Goatanti-Mouse IgG-HRP as the secondary antibody. Immunohistochemistry on Frozen Sections (1,5).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative in 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Nitrotyrosine

Target: Nitrotyrosine

Substanzklasse: Chemical

Hintergrund: Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an mportant component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, sinophilperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitro tyrosine-containing proteins therefore has shown high orrelation to disease states such as atherosclerosis, Alzheimer' disease, Parkinson's disease and amyotrophic lateral sclerosis (4).Synonyms: NO-Tyrosine, Nitro-Tyrosine