COL3A1 Antikörper (Chain alpha 1)

Produktnummer ABIN7581223

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch COL3A1 in WB, IF, IHC (p), IHC (fro), EIA und RIA. Es zeigt Reaktivität gegenüber Proben von Maus und Ratte.

Kurzübersicht für COL3A1 Antikörper (Chain alpha 1) (ABIN7581223)

Target: COL3A1 (Collagen, Type III, alpha 1 (COL3A1))

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser COL3A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA)

Produktdetails anti-COL3A1 Antikörper

Bindungsspezifität: Chain alpha 1

Spezifität: Rat Collagen type III 100 % . Rat Collagen I < 0.1 % Rat Collagen type V < 10 % Rat Elastin < 0.1 % (determined by solid phase RIA at 1/50 dilution).

Aufreinigung: Affinity Chromatography

Immunogen: Purified Collagen type III from Rat skin.

Anwendungsinformationen

Applikationshinweise: RIA. Western blot. ELISA: >/= 1/200. Immunflourescence: 1/80. Immunohistochemistry on Frozen and Paraffin Embedded Tissues: >/= 1/500. Pretreatment: After de-waxing the tissue slices are treated with 0.2 % hyaluronidase (app.300 U/mg) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blockingserum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBSfor 30 min at RT is recommended. Incubation Time: 60 minutes at RT or 2-8 °C over night. Positive Control: Rat skin or liver.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.5 mL distilled water. For further dilutions use appropriate antibody diluent.

Konzentration: 1.0 mg/mL

Buffer: PBS without BSA or preservatives

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Details zu COL3A1

Target: COL3A1 (Collagen, Type III, alpha 1 (COL3A1))

Hintergrund: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type III collagen is an alpha1(III)-trimer, MW 95 kDa, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and vitreous body.Synonyms: COL3A1

Gen-ID: 12825

NCBI Accession: NP_034060

UniProt: P08121

Pathways: Autophagie, Growth Factor Binding