P4HB Antikörper (AA 409-509) (Atto 390)

Produktnummer ABIN2486765

Dieses Anti-P4HB-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von P4HB in WB, IHC, IP, IF und ICC. Geeignet für Ratte.

Kurzübersicht für P4HB Antikörper (AA 409-509) (Atto 390) (ABIN2486765)

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser P4HB Antikörper ist konjugiert mit Atto 390

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-P4HB Antikörper (Atto 390)

Bindungsspezifität: AA 409-509

Spezifität: Detects ~58 kDa.

Kreuzreaktivität: Rind (Kuh), Hund, Meerschweinchen, Hamster, Human, Mollusca, Maus, Schwein, Ratte, Schaf, Xenopus laevis

Aufreinigung: Peptide Affinity Purified

Immunogen: Rat PDI synthetic peptide (409-509 aa C-terminal) conjugated to KLH

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Kommentare:

A 1:1000 dilution of ABIN2486765 was sufficient for detection of PDI in 20 μg of HeLa cell lysate by ECL immunoblot analysis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu P4HB

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Andere Bezeichnung: PDI

Hintergrund: The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation via its oxidase activity and isomerization via its isomerase activity, as well as the reduction of disulphide bonds in proteins (1). Studies suggest BiP and PDI work together sequentially to increase oxidation of these proteins (2, 3). PDI has also been found to function as a chaperone to prevent the aggregation of unfolded substrates, and serves as a subunit of prolyl 4-hydroxylase and microsomal triglyceride transferase (4, 5). PDI is an abundant 55 kDa protein located primarily in the ER, however studies have also proved its presence in the cytosol (1). PDI has the ability to reside in the ER permanently due to the highly conserved KDEL sequence at its carboxy-terminus (6). It uses carboxy-terminal KDEL as a retention signal, and this appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor (7).

Gen-ID: 287164

NCBI Accession: NP_001099245

UniProt: P04785

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism