P4HB Antikörper (AA 52-63) (PerCP)
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- Target Alle P4HB Antikörper anzeigen
- P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))
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Bindungsspezifität
- AA 52-63
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser P4HB Antikörper ist konjugiert mit PerCP
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Applikation
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Spezifität
- Detects ~48 kDa.
- Kreuzreaktivität
- Human
- Aufreinigung
- Protein G Purified
- Immunogen
- Synthesized peptide - 12 amino acids long (ADGEDGQDPHSK) corresponding to residues 52 - 63 of the EndoPDI protein
- Klon
- 2E7-7
- Isotyp
- IgG2a
- Top Product
- Discover our top product P4HB Primärantikörper
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- Applikationshinweise
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- WB (1:200)
- optimal dilutions for assays should be determined by the user.
- Kommentare
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1 μg/ml of ABIN2484906 was sufficient for detection of EndoPDI in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti -mouse IgG:HRP as the secondary antibody.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Conjugated antibodies should be stored at 4°C
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- Target
- P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))
- Andere Bezeichnung
- PDI (P4HB Produkte)
- Hintergrund
- Endothelial protein disulfide isomerase (EndoPDI) is a thioredoxin member of the protein disulfide isomerase family of chaperones. This enzyme has been localized to the endoplasmic reticulum in primary endothelial cells, but colocalizes with nucleoli in the nuclei of breast, colon, and renal cancer cells. EndoPDI mRNA and protein expression is induced by hypoxia and exhibits a protective effect on endothelial cells during hypoxia (1).
- Gen-ID
- 81567
- NCBI Accession
- NP_001139021
- UniProt
- Q8NBS9
- Pathways
- Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism
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