SOD3 Antikörper (Atto 594)

Produktnummer ABIN2484263

Dieses Anti-SOD3-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von SOD3 in WB, IHC, ELISA, ICC und IF. Geeignet für Human.

Kurzübersicht für SOD3 Antikörper (Atto 594) (ABIN2484263)

Target: SOD3 (Superoxide Dismutase 3, Extracellular (SOD3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SOD3 Antikörper ist konjugiert mit Atto 594

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Klon: 4GG11G6

Produktdetails anti-SOD3 Antikörper (Atto 594)

Spezifität: Detects extracellular SOD ~35 kDa.

Kreuzreaktivität: Meerschweinchen, Human, Maus, Ratte

Aufreinigung: Protein G Purified

Immunogen: Human extracellular SOD purified from aortas

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• IHC (1:100)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml of ABIN2484263 was sufficient for detection of EC-SOD in 20 μg of human cartilage lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu SOD3

Target: SOD3 (Superoxide Dismutase 3, Extracellular (SOD3))

Andere Bezeichnung: SOD3

Hintergrund: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (6). SOD3 can also be distinguished by its heparin-binding capacity (1).

Gen-ID: 6649

NCBI Accession: NP_003093

UniProt: P08294