GFP Antikörper (full length)

Produktnummer ABIN2467764

Dieses Anti-GFP-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von GFP in WB, ELISA und IHC. Geeignet für Aequorea victoria.

Kurzübersicht für GFP Antikörper (full length) (ABIN2467764)

Target: GFP (Green Fluorescent Protein (GFP))

Reaktivität: Aequorea victoria

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GFP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-GFP Antikörper

Bindungsspezifität: full length

Aufreinigung: Anti-GFP Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins.

Immunogen: Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Anwendungsinformationen

Applikationshinweise: Polyclonal anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. Recommended dilutions are as follows, ELISA 1:15,000 - 1:60,000, Western Blot 1:500 - 1:5,000, Immunohistochemistry 1:200 - 1:3,000. (Optimal dilutions should be determined by user).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01 % (w/v) Sodium Azide, pH 7.2

Handhabung: Avoid freeze thaw cycles

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 C or below prior to opening. Dilute only prior to immediate use. Forextended storage, aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product.

Details zu GFP

Target: GFP (Green Fluorescent Protein (GFP))

Andere Bezeichnung: GFP

Gen-ID: 7011691

NCBI Accession: YP_002302326

UniProt: P42212