HNF4A Antikörper (N-Term)
Kurzübersicht für HNF4A Antikörper (N-Term) (ABIN2465821)
Target
Alle HNF4A Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- N-Term
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Spezifität
- This antibody is expected to recognize the reported isoforms a, b and c (NP_849180.1, NP_000448.3, NP_849181.1 resp.).
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Aufreinigung
- HNF4A antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Immunogen
- HNF4A antibody was raised against a 14 amino acid synthetic peptide near the N-Terminus of HNF4A.
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Applikationshinweise
- ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 52 kDa and 48 kDa bands observed in lysates of human hepatocellular carcinoma cell line HepG2 (calculated MW of 52.8 kDa according to NP_000448.3 and 46.6 kDa according to NP_849181.1). Recommended concentration: 0.1-0.3 μg/mL.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 500 μg/mL
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Buffer
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- Minimize freezing and thawing.
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Lagerung
- -20 °C
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Informationen zur Lagerung
- Aliquot and store at -20 °C.
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- HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))
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Andere Bezeichnung
- HNF4A
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Gen-ID
- 3172
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NCBI Accession
- NP_849180, NP_000448, NP_849181
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UniProt
- P41235
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Pathways
- AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process
Target
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