NPT (truncated) Antikörper

Produktnummer ABIN2464088

Der Maus Monoklonal Anti--Antikörper wurde für ELISA und WB validiert. Er ist geeignet, in Proben von Human zu detektieren.

Kurzübersicht für NPT (truncated) Antikörper (ABIN2464088)

Target: NPT

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: ELISA, Western Blotting (WB)

Klon: 4B4D1

Produktdetails

Bindungsspezifität: truncated

Aufreinigung: Antibody is purified by protein A affinity chromatography.

Immunogen: Ni-NTA purified truncated recombinant NPT expressed in E. Coli strain BL21 (DE3).

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Western Blot:Dilution 1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Lagerung: -20 °C

Informationen zur Lagerung: NPT monoclonal antibody can be stored at -20 °C, stable for one year.

Antigendetails

Target: NPT

Hintergrund: Neomycin phosphotransferase II (nptII) gene is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph(3')-II or NPTII) enzyme. NPTII is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents.

Gen-ID: 11979140

UniProt: Q79C30