NPT Antikörper

Produktnummer ABIN966694

Produktdetails

Target: NPT

Reaktivität: Human

Wirt: Bitte anfragen

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: ELISA, Western Blotting (WB)

Spezifität: Ni-NTA purified truncated recombinant NPT expressed in E. Coli strain BL21 (DE3)

Aufreinigung: Antibodies are purified by protein A affinity chromatography

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Western Blot: Dilution 1: 500- 1: 2,000
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Referenzen

Stewart, Richards, Halfhill: "Transgenic plants and biosafety: science, misconceptions and public perceptions." in: BioTechniques, Vol. 29, Issue 4, pp. 832-6, 838-43, (2001) (PubMed).

Antigendetails

Target: NPT

Hintergrund: Neomycin phosphotransferase II (nptII) gene is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph(3')-II or NPTII) enzyme. NPTII is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents.