NPT Antikörper

Produktnummer ABIN969318

Produktdetails

Target: NPT

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: ELISA, Western Blotting (WB)

Aufreinigung: purified

Immunogen: Purified recombinant fragment of NPT expressed in E. coli.

Klon: 4B4D1

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: ELISA: 1:10000, WB: 1:500 - 1:2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: 4°C, -20°C for long term storage

Referenzen

Lukasik, Bradley, Scott, Dea, Koo, Hsu, Bartz, Farrah: "Reduction of poliovirus 1, bacteriophages, Salmonella montevideo, and Escherichia coli O157:H7 on strawberries by physical and disinfectant washes." in: Journal of food protection, Vol. 66, Issue 2, pp. 188-93, (2003) (PubMed).

Stewart, Richards, Halfhill: "Transgenic plants and biosafety: science, misconceptions and public perceptions." in: BioTechniques, Vol. 29, Issue 4, pp. 832-6, 838-43, (2001) (PubMed).

Antigendetails

Target: NPT

Hintergrund:

Description: Neomycin phosphotransferase II (nptII) gene is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph(3')-II or NPTII) enzyme. NPTII is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents.

Aliases: N/A