GFP Antikörper (full length)

Produktnummer ABIN2464056

Dieses Anti-GFP-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von GFP in WB und ELISA. Geeignet für Aequorea victoria.

Kurzübersicht für GFP Antikörper (full length) (ABIN2464056)

Target: GFP (Green Fluorescent Protein (GFP))

Reaktivität: Aequorea victoria

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GFP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Klon: 4B10B2

Produktdetails anti-GFP Antikörper

Bindungsspezifität: full length

Immunogen: Ni-NTA purified full-length recombinant GFP-His expressed in E. Coli strain BL21 (DE3).

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Western Blot:1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified antibody in PBS containing 0.03 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Lagerung: -20 °C

Informationen zur Lagerung: GFP monoclonal antibody can be stored at -20 °C, stable for one year.

Details zu GFP

Target: GFP (Green Fluorescent Protein (GFP))

Andere Bezeichnung: GFP

Hintergrund: GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.

Gen-ID: 7011691

NCBI Accession: YP_002302326

UniProt: B6UPG7