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RFC5 Antikörper

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch RFC5 in WB, IHC und ELISA. Er zeigt eine Reaktivität gegenüber Human und Hund.
Produktnummer ABIN2462953

Kurzübersicht für RFC5 Antikörper (ABIN2462953)

Target

Alle RFC5 Antikörper anzeigen
RFC5 (Replication Factor C (Activator 1) 5, 36.5kDa (RFC5))

Reaktivität

  • 14
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Hund

Wirt

  • 9
  • 3
  • 2
Kaninchen

Klonalität

  • 12
  • 2
Polyklonal

Konjugat

  • 14
Dieser RFC5 Antikörper ist unkonjugiert

Applikation

  • 14
  • 5
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA
  • Aufreinigung

    Antibody is purified by peptide affinity chromatography method.

    Immunogen

    Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RFC5.
  • Applikationshinweise

    RFC5 antibody can be used for detection of RFC5 by ELISA at 1:62500. RFC5 antibody can be used for detection of RFC5 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

    Konzentration

    1 mg/mL

    Buffer

    Antibody is lyophilized in PBS buffer with 2 % sucrose.

    Handhabung

    As with any antibody avoid repeat freeze-thaw cycles.

    Lagerung

    4 °C/-20 °C

    Informationen zur Lagerung

    For short periods of storage (days) store at 4 °C. For longer periods of storage, store RFC5 antibody at -20 °C.
  • Target

    RFC5 (Replication Factor C (Activator 1) 5, 36.5kDa (RFC5))

    Andere Bezeichnung

    RFC5

    Hintergrund

    The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. RFC5 is the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system.The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

    Molekulargewicht

    38 kDa

    Gen-ID

    5985

    NCBI Accession

    NP_031396

    UniProt

    P40937

    Pathways

    Telomere Maintenance, DNA Reparatur, DNA Replication, Synthesis of DNA
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