PARP1 Antikörper

Produktnummer ABIN2460801

Dieses Anti-PARP1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PARP1 in WB und ELISA. Geeignet für Human, Maus, Ratte und Hund.

Kurzübersicht für PARP1 Antikörper (ABIN2460801)

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PARP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-PARP1 Antikörper

Aufreinigung: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PARP1.

Anwendungsinformationen

Applikationshinweise: PARP1 antibody can be used for detection of PARP1 by ELISA at 1:62500. PARP1 antibody can be used for detection of PARP1 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Konzentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handhabung: As with any antibody avoid repeat freeze-thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: For short periods of storage (days) store at 4 °C. For longer periods of storage, store PARP1 antibody at -20 °C.

Details zu PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Andere Bezeichnung: PARP1

Hintergrund: PARP1 encodes a chromatin-associated enzyme, poly (ADP-ribosyl)transferase, which modifies various nuclear proteins by poly (ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. PARP1 can be cleaved resulting in fragements of 29 and 85 kDa.

Molekulargewicht: 113 kDa

Gen-ID: 57097

NCBI Accession: NP_001609

UniProt: P09874

Pathways: Apoptose, Caspase Kaskade in der Apoptose, DNA Reparatur, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location