SUMO1 Antikörper (full length) (Biotin)

Produktnummer ABIN2452137

Zitiert in 4+ Publikationen. Der Biotin-konjugierte Ratte Monoklonal anti-SUMO1 Antikörper (Klon 4D12) (ABIN2452137) detektiert spezifisch SUMO1 in WB, IF, ELISA und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für SUMO1 Antikörper (full length) (Biotin) (ABIN2452137)

Target: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Reaktivität: Human

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser SUMO1 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 4D12

Produktdetails anti-SUMO1 Antikörper (Biotin)

Bindungsspezifität: full length

Spezifität: Specific to human, simian, mouse and rat SUMO1.

Kreuzreaktivität (Details): Not tested for other species

Aufreinigung: The antibody was produced in serum-free medium and purified by proprietary chromatography procedures under mild conditions and conjugated with biotin.

Sterilität: Sterile filtered

Immunogen: Recombinant GST-fused human SUMO1 (full length)

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: 1. Western blotting: 1/1,000 dilution
2. Immunofluorescence staining: 1/100 dilution
3. Immunohistochemistry,frozen section: 1/100 dilution
4. ELISA (assay dependent)
Other applications have not been tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS (x1), 50 % glycerol. Azide and carrier free.

Konservierungsmittel: Azide free

Lagerung: -20 °C

Referenzen für anti-SUMO1 Antikörper (Biotin) (ABIN2452137)

Ulrich: "The fast-growing business of SUMO chains." in: Molecular cell, Vol. 32, Issue 3, pp. 301-5, (2008) (PubMed).

Saitoh, Uchimura, Tachibana, Sugahara, Saitoh, Nakao: "In situ SUMOylation analysis reveals a modulatory role of RanBP2 in the nuclear rim and PML bodies." in: Experimental cell research, Vol. 312, Issue 8, pp. 1418-30, (2006) (PubMed).

Uchimura, Ichimura, Uwada, Tachibana, Sugahara, Nakao, Saitoh: "Involvement of SUMO modification in MBD1- and MCAF1-mediated heterochromatin formation." in: The Journal of biological chemistry, Vol. 281, Issue 32, pp. 23180-90, (2006) (PubMed).

Cheng, Bawa, Lee, Gong, Yeh: "Role of desumoylation in the development of prostate cancer." in: Neoplasia (New York, N.Y.), Vol. 8, Issue 8, pp. 667-76, (2006) (PubMed).

Details zu SUMO1

Target: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Andere Bezeichnung: SUMO1

Hintergrund: SUMO (Small Ubiquitin-like Modifier) proteins are a family of small proteins that are covalently attached to and detached from other proteins in cells to modify their function. Unlike ubiquitination, which targets proteins for degradation, SUMO modification plays a critical role in a number of cellular functions including nucleocytoplasmic transport, gene expression, cell cycle and formation of subnuclear structures such as promyelocytic leukemia (PML) bodies. There are three confirmed SUMO isoforms in human, SUMO1, SUMO2 and SUMO3. SUMO2 /3 show a high degree of similarity to each other and are distinct from SUMO1. Individual SUMO family members are all targeted to different proteins with diverse biological functions. SUMO-1 is conjugated to RanGAP, PML, p53 and IkappaB-alpha to regulate nuclear trafficking, formation of subnuclear structures, regulation of transcriptional activity and protein stability. SUMO1 is encoded as a 101 aa protein and first Met and C-terminal 4 aa are removed from the preprotein.

UniProt: P63165

Pathways: M Phase, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, Ubiquitin Proteasome Pathway