Rad6 (full length) Antikörper

Produktnummer ABIN2452101

Produktdetails

Target: Rad6

Bindungsspezifität: full length

Reaktivität: Human, Maus, Ratte, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Kreuzreaktivität (Details): Not tested in other species.

Aufreinigung: Affinity purified with the immunogen conjugated to agarose

Sterilität: Sterile filtered

Immunogen: Human Rad6B full-length recombinant protein

Anwendungsinformationen

Applikationshinweise: 1) Western blotting: 1,000~2,000 fold dilution
2) Immunoprecipitation (assay dependent)
3) Immunofluoscent staining (assay dependent)
Other applications have not been tested

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS (x1), 50 % glycerol. Azide and carrier free.

Konservierungsmittel: Azide free

Lagerung: -20 °C

Informationen zur Lagerung: Upon arrival centrifuge briefly and store at -20 C.

Referenzen

Masuyama, Tateishi, Yomogida, Nishimune, Suzuki, Sakuraba, Inoue, Ogawa, Yamaizumi: "Regulated expression and dynamic changes in subnuclear localization of mammalian Rad18 under normal and genotoxic conditions." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 10, Issue 8, pp. 753-62, (2005) (PubMed).

Antigendetails

Target: Rad6

Hintergrund: Resume of DNA synthesis, which was halted by various physical and chemical factors, was triggered by repair system including Rad6. Rad6 (152a.a, 17.3 kDa) is the ubiqutin-conjugated enzyme E2A, and complex with two Rad18 Molecules, ubiquitin ligase E3, to ubiquitinate PCNA by utilizing poly-ubiquitin chain that HLTF-UBC13 synthesized and they are involved in translesion DNA synthesis (TLS) and template switch (TS). Two isoforms of Rad6, Rad6A and Rad6B differs in only 6 amino acids. They also play an important role in epigenetic transcriptional regulation by catalyzing the monoubiquitination of histone H2B at Lys-120.

UniProt: P49459, P63146