DYKDDDDK Tag Antikörper (C-Term, N-Term)

Produktnummer ABIN2443789

Dieser Maus Monoklonal Antikörper detektiert spezifisch DYKDDDDK Tag in WB, ELISA, IP, IHC und DB.

Kurzübersicht für DYKDDDDK Tag Antikörper (C-Term, N-Term) (ABIN2443789)

Target: DYKDDDDK Tag

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser DYKDDDDK Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Dot Blot (DB)

Klon: FG4R

Produktdetails anti-DYKDDDDK Tag Antikörper

Bindungsspezifität: C-Term, N-Term

Verwendungszweck: FLAG Tag Mouse Monoclonal Antibody (FG4R)

Sequenz: DYKDDDDK

Spezifität: The antibody recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins.

Aufreinigung: Protein G purified

Immunogen: The antibody was raised against DYKDDDDK (FLAG) synthetic peptide conjugated to KLH.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: WB 1:1000,IHC 1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: If reconstituted with deionized water in 100 μL: WB 1:1000-3,000, IHC 1:500-2,000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Haltbarkeit: 24 months

Details zu DYKDDDDK Tag

Target: DYKDDDDK Tag

Andere Bezeichnung: FLAG Tag

Substanzklasse: Tag

Hintergrund: Epitope tags have application in the labeling, isolation and detection of proteins using immunoblotting, Immunoprecipitation and immunostaining techniques. Epitope tags can be used, by affinity chromatography, to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. Due its small size the protein's biochemical properties seem not to be affected by the tag protein. FLAG-tag, or FLAG octapeptide, a polypeptide consisting of eight amino acids (DYKDDDDK) can be added to a protein using recombinant DNA technology, therefore, the tag can be detected with the fused protein in many different assays that require recognition by an antibody.