CK1 epsilon Antikörper (C-Term)

Produktnummer ABIN238540

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch CK1 epsilon in WB, ELISA, IHC, IF und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CK1 epsilon Antikörper (C-Term) (ABIN238540)

Target: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CK1 epsilon Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-CK1 epsilon Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: CSNK1E

Sequenz: PASQTSVPFD HLGK

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-PASQTSVPFDHLGK, from the C Terminus of the protein sequence according to NP_001885.1, NP_689407.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 5 μg/mL.

Western Blot: Approx 48 kDa band observed in nuclear lysates of cell lines A431and HeLa, and approx 45 kDa in nuclear lysates of cell line K562 (calculated MW of 47.3 kDa according to NP_001276841.1). Recommended concentration: 0.3-1 μg/mL. Primary incubatio

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu CK1 epsilon

Target: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Andere Bezeichnung: CSNK1E

Hintergrund: CSNK1E, casein kinase 1, epsilon, RP1-5O6.1, HCKIE, MGC10398 , CKI-epsilon, OTTHUMP00000063258, OTTHUMP00000063263, casein kinase 1 epsilon, casein kinase I epsilon

Gen-ID: 1454, 27373, 58822

NCBI Accession: NP_001276841

Pathways: Hedgehog Signalweg, M Phase