Integrin beta 2 Antikörper (Activation Site) (APC)

Produktnummer ABIN238446

Zitiert in 1 Publikation. Der APC-konjugierte Maus Monoklonal anti-Integrin beta 2 Antikörper (Klon MEM-148) (ABIN238446) detektiert spezifisch Integrin beta 2 in FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Integrin beta 2 Antikörper (Activation Site) (APC) (ABIN238446)

Target: Integrin beta 2 (ITGB2)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Integrin beta 2 Antikörper ist konjugiert mit APC

Applikation: Flow Cytometry (FACS)

Klon: MEM-148

Produktdetails anti-Integrin beta 2 Antikörper (APC)

Bindungsspezifität: Activation Site

Spezifität: The antibody MEM-148 recognizes the epitope exposed on free human CD18 chains unassociated with CD11 as well as on high-affinity state of LFA-1. CD18 (integrin β2 subunit, β2 integrin) is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.

Aufreinigung: Size-exclusion chromatography.

Immunogen: Peripheral blood mononuclear cells.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Suitable for Flow Cytometry analysis of human blood cells using 20 μL reagent / 100 μL ofwhole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 15 mM sodium azide as preservative and 0.2 % (w/v) high-grade BSA (Protease free) as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2-8 °C for one month or at -20 °C for longer.

Referenzen für anti-Integrin beta 2 Antikörper (APC) (ABIN238446)

Aubert, Yoon, Sloan, Spear, Jerome: "The virological synapse facilitates herpes simplex virus entry into T cells." in: Journal of virology, Vol. 83, Issue 12, pp. 6171-83, (2009) (PubMed).

Details zu Integrin beta 2

Target: Integrin beta 2 (ITGB2)

Andere Bezeichnung: CD18 / ITGB2

Hintergrund: CD18, integrin β2 subunit, forms heterodimers with four types of CD11 Molecule to constitute leukocyte (β2) integrins: αLβ2 (CD11a/CD18, LFA-1), αMβ2 (CD11b/CD18, Mac-1, CR3), αXβ2 (CD11c/CD18) and αDβ2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7

Gen-ID: 3689

NCBI Accession: NP_000202

UniProt: P05107

Pathways: NF-kappaB Signalweg, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation