LIME Antikörper (AA 141-295) (PE)

Produktnummer ABIN238427

Der Maus Monoklonal anti-LIME Antikörper wird verwendet zum Nachweis von LIME in Proben von Human. Er wurde validiert für FACS.

Kurzübersicht für LIME Antikörper (AA 141-295) (PE) (ABIN238427)

Target: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser LIME Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS)

Klon: LIME-06

Produktdetails anti-LIME Antikörper (PE)

Bindungsspezifität: AA 141-295

Spezifität: The antibody LIME-06 was raised against intracellular fragment corresponding to aa 141-295 of human LIME, a 31 kDa Lck-interacting transmembrane adaptor expressed by T cells.

Aufreinigung: Size-exclusion chromatography.

Immunogen: Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Suitable for Flow Cytometry analysis (5 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS containing 15 mM sodium azide as preservative and 0.2 % (w/v) high-grade BSA (Protease free) as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody in the dark at 2-8 °C. Do Not Freeze! Avoid prolonged exposure to light.
Shelf Life: one year from despatch.

Haltbarkeit: 12 months

Details zu LIME

Target: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Andere Bezeichnung: LIME1

Hintergrund: LIME (Lck-interacting molecule) is a 31 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.Synonyms: LIME, LIME-1, LIME1, Lck-interacting membrane protein, Lck-interacting molecule, Lck-interacting transmembrane adapter 1

Gen-ID: 54923

UniProt: Q9H400