LIME Antikörper (AA 141-295)

Produktnummer ABIN94424

Es gibt 1 Publikation für dieses Produkt. Der Maus Monoklonal anti-LIME Antikörper wird verwendet zum Nachweis von LIME in Proben von Human. Er wurde validiert für IP und ICFC.

Kurzübersicht für LIME Antikörper (AA 141-295) (ABIN94424)

Target: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser LIME Antikörper ist unkonjugiert

Applikation: Immunoprecipitation (IP), Intracellular Flow Cytometry (ICFC)

Klon: LIME-06

Produktdetails anti-LIME Antikörper

Bindungsspezifität: AA 141-295

Verwendungszweck: Anti-LIME1 Purified

Kreuzreaktivität (Details): Human

Produktmerkmale: The antibody LIME-06 was raised against intracellular fragment corresponding to aa 141-295 of human LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 8-12 μg/mL. Intracellular staining.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-LIME Antikörper (ABIN94424)

Brdicková, Brdicka, Angelisová, Horváth, Spicka, Hilgert, Paces, Simeoni, Kliche, Merten, Schraven, Horejsí: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, (2003) (PubMed).

Details zu LIME

Target: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Andere Bezeichnung: LIME1

Hintergrund: Lck interacting transmembrane adaptor 1,LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.,LITA1

Gen-ID: 54923

UniProt: Q9H400