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ROCK2 Antikörper (pTyr256)

Dieses Anti-ROCK2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von ROCK2 in WB, ELISA und IHC. Geeignet für Human. Dieses Primary Antibody wurde in 1 Publikation zitiert.
Rockland
Produktnummer ABIN233829
Hersteller Produkt- Nr.: 600-401-998

Kurzübersicht für ROCK2 Antikörper (pTyr256) (ABIN233829)

Target

Alle ROCK2 Antikörper anzeigen
ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

Reaktivität

  • 97
  • 55
  • 54
  • 4
  • 4
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
Human

Wirt

  • 91
  • 8
  • 1
Kaninchen

Klonalität

  • 87
  • 13
Polyklonal

Konjugat

  • 55
  • 5
  • 5
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser ROCK2 Antikörper ist unkonjugiert

Applikation

  • 82
  • 37
  • 27
  • 27
  • 23
  • 18
  • 17
  • 14
  • 11
  • 9
  • 3
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
  • Bindungsspezifität

    • 15
    • 15
    • 9
    • 8
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr256

    Hersteller Produkt- Nr.

    600-401-998

    Hersteller

    Rockland

    Verwendungszweck

    Rock-2 phospho Y256 Antibody

    Kreuzreaktivität (Details)

    This antibody is specific for human ROCK-2 protein phosphorylated at Y256.

    Produktmerkmale

    Synonyms: rabbit anti-ROCK2 antibody, Rho-associated protein kinase 2, Rho-associated coiled-coil-containing protein kinase 2, p164 ROCK-2, Rho kinase 2, Rho-associated coiled-coil-containing protein kinase II, ROCK-II, ROCK-2, ROCK 2

    Aufreinigung

    This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against nonphospho-peptide.

    Sterilität

    Sterile filtered

    Immunogen

    Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y256 of human ROCK-2.

    Immunogen Type: Conjugated Peptide

    Isotyp

    IgG
  • Applikationshinweise

    Immunohistochemistry Dilution: User Optimized

    Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 164 kDa in size corresponding to phosphorylated ROCK-2 by western blotting in the appropriate cell lysate or extract.  This phospho-specific polyclonal antibody reacts with human ROCK-2 pY256 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

    Western Blot Dilution: 1:200 - 1:2,000

    ELISA Dilution: 1:2,000 - 1:20,000

    Other: User Optimized

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1.0 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Haltbarkeit

    12 months
  • Akhtar, Yousif, Dhaunsi, Sarkhouh, Chandrasekhar, Attur, Benter: "Activation of ErbB2 and Downstream Signalling via Rho Kinases and ERK1/2 Contributes to Diabetes-Induced Vascular Dysfunction." in: PLoS ONE, Vol. 8, Issue 6, pp. e67813, (2017) (PubMed).

  • Target

    ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

    Andere Bezeichnung

    ROCK2

    Hintergrund

    Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Progression of tumors to invasive and metastatic forms requires that tumor cells undergo dramatic morphological changes, a process regulated by Rho GTPases.  Increased levels of RhoA or RhoC, as well as the Rho effector proteins ROCK-1 or ROCK-2, were observed in various human carcinomas. In vivo studies have also shown that ROCK inhibition reduced the invasiveness of several tumor cell lines. It is suggested that Rho and ROCK signaling contribute to the morphological changes and metastatic behavior of some tumor cells. Thus, elevated Rho and ROCK expression is associated with tumorigenesis, particularly during the progression to invasive and metastatic phenotypes. Phosphorylation of certain residues in ROCK-2 may be critical for its kinase activity.

    Gen-ID

    9475, 4520225

    UniProt

    O75116

    Pathways

    Microtubule Dynamics, WNT Signalweg, Tube Formation
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