ENOS Antikörper (pSer1179)

Produktnummer ABIN224813

Produktdetails anti-ENOS Antikörper

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Bindungsspezifität: pSer1179

Reaktivität: Human, Ratte, Maus, Schwein, Rind (Kuh), Hund, Meerschweinchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ENOS Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Spezifität: Recognizes bovine eNOS protein, phosphorylated at Ser1179 (~135kD). Reactivity has been confirmed with VEGF-stimulated, serum-starved BAEC (bovine aortic endothelial cell) lysates. A very low signal was observed in the lysates of serum-starved, unstimulated BAEC and no signal was observed in lysates of unstimulated BAEC in the presence of serum. Species sequence homology: human, rat, mouse, canine, porcine, and guinea pig.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the C-terminal region of the bovine eNOS (endothelial nitric oxide synthase, NOS III) protein, phosphorylated at Ser1179.

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.1 - 1 μg/mL), IF, WB (1 - 3 μg/mL)

Usage: Suitable for use in Immunofluorescence, ELISA and Western Blot. ELISA: 0.1-1 μg/mL. Western Blot: 1-3 μg/mL. Immunofluorescence: reactive with monkey COS cells in immunofluorescence assays (per Fulton D, et al. 2002 publication.).

Kommentare:

Target Species of Antibody: Bovine

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu ENOS

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Andere Bezeichnung: NOS3 / eNOS (NOS3 Produkte)

Synonyme: ECNOS antikoerper, eNOS antikoerper, 2310065A03Rik antikoerper, Nos-3 antikoerper, ecNOS antikoerper, EC-NOS antikoerper, NOSIII antikoerper, cNOS antikoerper, NOS antikoerper, NOS3 antikoerper, eNos antikoerper, ENOS antikoerper, nitric oxide synthase 3 antikoerper, nitric oxide synthase 3, endothelial cell antikoerper, NOS3 antikoerper, Nos3 antikoerper, nos3 antikoerper

Hintergrund: Name/Gene ID: NOS3

Synonyms: NOS3, Constitutive NOS, CNOS, Endothelial NOS, ECNOS, NOS type III, NOSIII, EC-NOS, ENOS, Nitric oxide synthase 3

Gen-ID: 4846

UniProt: P29474

Pathways: ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling