CD46 Antikörper

Produktnummer ABIN2192126

Der Maus Monoklonal Anti-CD46-Antikörper wurde für WB, IF und IA validiert. Er ist geeignet, CD46 in Proben von Ratte zu detektieren. Es sind 4+ Publikationen verfügbar.

Kurzübersicht für CD46 Antikörper (ABIN2192126)

Target: CD46

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD46 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoassay (IA)

Klon: MM-1

Produktdetails anti-CD46 Antikörper

Sterilität: 0.2 μm filtered

Anwendungsinformationen

Applikationshinweise: For immunohistology, immunofluorescence and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Positive Western blotting: rat testis lysate, non-reducing conditions, band size 38 kDa (Ref. 1and 4) control

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Haltbarkeit: 12 months

Referenzen für anti-CD46 Antikörper (ABIN2192126)

Mizuno, Harris, Morgan: "Spermatogenic cells distal to the blood-testis barrier in rats lack C3 convertase regulators and may be at risk of complement-mediated injury." in: Journal of reproductive immunology, Vol. 69, Issue 1, pp. 23-34, (2006) (PubMed).

Mizuno, Donev, Harris, Morgan: "CD55 in rat male reproductive tissue: differential expression in testis and expression of a unique truncated isoform on spermatozoa." in: Molecular immunology, Vol. 44, Issue 7, pp. 1613-22, (2006) (PubMed).

Mizuno, Harris, Suzuki, Matsuo, Morgan: "Expression of CD46 in developing rat spermatozoa: ultrastructural localization and utility as a marker of the various stages of the seminiferous tubuli." in: Biology of reproduction, Vol. 72, Issue 4, pp. 908-15, (2005) (PubMed).

Mizuno, Harris, Johnson, Morgan: "Rat membrane cofactor protein (MCP; CD46) is expressed only in the acrosome of developing and mature spermatozoa and mediates binding to immobilized activated C3." in: Biology of reproduction, Vol. 71, Issue 4, pp. 1374-83, (2004) (PubMed).

Details zu CD46

Target: CD46

Andere Bezeichnung: CD46

Hintergrund: The monoclonal antibody MM.1 recognizes CD46, also known as membrane cofactor protein. An immunohistological survey of rat tissues revealed that CD46 is expressed only in the testis and epididymis of adult rats more specifically, only by spermatozoa and late spermatozoal precursors. In contrast, in humans, CD46 is expressed on every cell and tissue, with the exception of erythrocytes. CD46 serves to down-regulate the activation of complement on host tissue. It functions as a cofactor by binding to C3b and C4b. Binding is permitted by factor I, a serine protease of plasma, to degrade C3b and C4b and serves to protect the host cell against autologous attack. In human, it also serves as a receptor for measles virus. Two isoforms of CD46 predominate and arise by alternative splicing of a single CD46 gene. The isoforms differ in the length. CD46 cDNA encodes a signal sequence followed by four complement control protein domains (also called short consensus repeats (SCR)). Aliases Membrane cofactor protein Immunogen Second and third SCR domains of MCP fused to IgG-Fc, MCP23-Ig

Pathways: Regulation of Actin Filament Polymerization